On the nature and differential distribution of mRNAs in hippocampal neurites: implications for neuronal functioning.
AUTOR(ES)
Miyashiro, K
RESUMO
Neurons are highly polarized cells with a mosaic of cytoplasmic and membrane proteins differentially distributed in axons, dendrites, and somata. In Drosophila and Xenopus, mRNA localization coupled with local translation is a powerful mechanism by which regionalized domains of surface or cytoplasmic proteins are generated. In neurons, there is substantial ultrastructural evidence positing the presence of protein synthetic machinery in neuronal processes, especially at or near postsynaptic sites. There are, however, remarkably few reports of mRNAs localized to these regions. We now present direct evidence that an unexpectedly large number of mRNAs, including members of the glutamate receptor family, second messenger system, and components of the translational control apparatus, are present in individual processes of hippocampal cells in culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=45113Documentos Relacionados
- Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization.
- Different mRNAs code for dopa decarboxylase in tissues of neuronal and nonneuronal origin.
- Visualizing the distribution and transport of mRNAs in living cells
- Differential expression of mRNAs for protein kinase inhibitor isoforms in mouse brain.
- Internal initiation of translation of five dendritically localized neuronal mRNAs