On the Process of Cellular Division in Escherichia coli, V. Incorporation of Deoxynucleoside Triphosphates by DNA Thermosensitive Mutants of Escherichia coli Also Lacking DNA Polymerase Activity*
AUTOR(ES)
Mordoh, José
RESUMO
Double mutants of Escherichia coli were constructed by combining DNA thermosensitive mutations affecting either DNA initiation (DnaA) or elongation (DnaB) with the Pol A1 mutation, which abolishes DNA polymerase activity. Incorporation of labeled deoxynucleoside triphosphates was studied in these mutants using toluene-treated cells. This incorporation was found to be exactly correlated with the capacity of the mutants to synthesize DNA in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283272Documentos Relacionados
- Process of Cellular Division in Escherichia coli: Physiological Study on Thermosensitive Mutants Defective in Cell Division
- Mutant of Escherichia coli with Thermosensitive Protein in the Process of Cellular Division
- On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein.
- On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins.
- On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli