On the pyrogenic action of intravenous lipid A in rabbits.
AUTOR(ES)
Kenedi, E
RESUMO
1. Previous evidence purporting to show that lipid A is the pyrogenic moiety of endotoxin is demonstrably inconclusive. 2. We have extracted lipid A from endotoxin of Salmonella typhosa and tested the pyrogenic action of the lipid A, the residual polysaccharide and the parent endotoxin, by intravenous injection in conscious rabbits. 3. Lipid A dissolved in an aqueous solution of rabbit serum albumin induced a significant pyrexia of short latency, while neither rabbit serum albumin alone, nor the polysaccharide from S. typhosa, affected body temperature. The physical presence in the injectate of the polysaccharide from S. typhosa did not enhance the pyrogenicity of the lipid. 4. Dose-response curves for lipid A and the parent endotoxin, over the dose range 10 ng-20 micrograms, showed that lipid A incorporated in endotoxin was much more pyrogenic than pure lipid A in solution. When separated from the polysaccharide component of endotoxin, lipid A lost more than 99.9% of its pyrogenic activity, at threshold doses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1225663Documentos Relacionados
- Effect of inflammation on intraocular penetration of intravenous ofloxacin in albino rabbits.
- Physiology of the potentiation of lethal endotoxin shock by streptococcal pyrogenic exotoxin in rabbits.
- Lipid A dependence of the ocular response to circulating endotoxin in rabbits.
- Effect of intravenous iron-dextran (Imferon) infusion on antigen induced monarticular arthritis in rabbits.
- Intravenous infusion of apolipoprotein E accelerates clearance of plasma lipoproteins in rabbits.