Optical recording of light-evoked calcium signals in the functionally intact retina
AUTOR(ES)
Denk, Winfried
FONTE
The National Academy of Sciences
RESUMO
Using two-photon excitation of fluorescent indicator dyes, we measured calcium concentration transients in retinal ganglion and amacrine cells without destroying the light sensitivity of the retina by maximally activating or bleaching the photoreceptors. This allowed an immediate assessment of the cellular morphology and study of the calcium signals evoked by visual stimuli. Calcium dynamics in individual dendritic processes could be examined for extensive periods without deterioration and with little apparent phototoxicity at excitation wavelengths of from 930 to 990 nm. Light-evoked increases in calcium were resolved in ganglion- and amacrine-cell neurites, making it possible to use optical recording to study the relationship between calcium signaling and retinal function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=22046Documentos Relacionados
- The relationship between light-evoked synaptic excitation and spiking behaviour of salamander retinal ganglion cells.
- Odor-evoked calcium signals in dendrites of rat mitral cells
- Optical Recording of Odor-Evoked Responses in the Olfactory Brain of the Naïve and Aversively Trained Terrestrial Snails
- Light-induced calcium release by intact retinal rods.
- RECORDING OF THE OCCIPITAL LOBE RESPONSE IN MAN AFTER LIGHT STIMULATION*
