Organization of actin cytoskeleton in normal and regenerating arterial endothelial cells.
AUTOR(ES)
Gabbiani, G
RESUMO
The distribution of actin stress fibers in normal and regenerating (after endothelial denudation by means of a balloon catheter) rabbit aortic endothelial cells has been studied by means of immunofluorescence with human actin autoantibodies on en face endothelial cell preparations. Our results show that: (i) under normal conditions actin is accumulated as a network at the periphery of endothelial cells. Stress fibers are present only in endothelial cells located immediately below intercostal artery branches; (ii) stress fibers develop in endothelial cells early during regeneration and persist after the end of endothelial mitotic and motile activities; and (iii) the orientation of stress fibers within the cytoplasm follows the direction of blood flow, with the exception of stress fibers situated in cells at the edge of the wound, when endothelial cell progression toward the denuded area as well as mitotic activity have ceased. We conclude that stress fibers are an organelle present in endothelial cells in vivo and that they reorganize during endothelial cell adaptation to unfavorable or pathological situations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393820Documentos Relacionados
- Relationship between organization of the actin cytoskeleton and the cell cycle in normal and adenovirus-infected rat cells.
- Patterns of organization of actin and myosin in normal and transformed cultured cells.
- Regulation of the Actin Cytoskeleton by Thrombin in Human Endothelial Cells: Role of Rho Proteins in Endothelial Barrier Function
- Neuroprotection mediated by changes in the endothelial actin cytoskeleton
- RhoE Regulates Actin Cytoskeleton Organization and Cell Migration