Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives.

AUTOR(ES)

Alul, R H

RESUMO

A procedure is described for linking nucleosides covalently to controlled pore glass or cross-linked polystyrene supports by means of an oxalyl anchor. Though stable to triethylamine and diisopropylamine, the nucleoside-oxalyl link can be cleaved within a few minutes at room temperature with ammonium hydroxide in methanol. This new anchor can be used in automated synthesis of conventional oligonucleotides. The primary value, however, is that it enables one to employ solid support methodology to synthesize a variety of base-sensitive oligonucleotide derivatives, as illustrated here by synthesis of oligomers with base protecting groups intact and with methyl phosphotriester groups at the internucleoside links.

Documentos Relacionados

• Evaluation of ascorbic acid in protecting labile folic acid derivatives.
• Synthesis and antimicrobial activity of a series of caespitin derivatives.
• Complementary addressed modification and cleavage of a single stranded DNA fragment with alkylating oligonucleotide derivatives.
• Synthesis of N2, N2, 7-trimethylguanosine cap derivatives.
• Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.