Oxidation of palmitic acid by Mycobacterium leprae in an axenic medium.
AUTOR(ES)
Franzblau, S G
RESUMO
The ability of Mycobacterium leprae to oxidize palmitic acid during incubation in an axenic medium was studied. By using a Buddemeyer-type detection system, partially purified nude-mouse-derived M. leprae was found to produce 14CO2 from 14C-labeled palmitic acid in a linear fashion for at least 1 week. Procedures known to remove residual host tissue did not diminish the rate of 14CO2 evolution, indicating that bacterial metabolism was being measured. Palmitate oxidation was temperature sensitive, with an apparent optimum of 33 degrees C, but pH insensitive. Bacilli exposed to a variety of antileprosy drugs for 1 or 2 weeks displayed significantly reduced rates of 14CO2 evolution upon subsequent addition of 14C-labeled palmitic acid. This activity could be readily detected with 10(6) bacilli, thus indicating its potential for use in clinical susceptibility testing.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266168Documentos Relacionados
- Growth and cholesterol oxidation by Mycobacterium species in Tween 80 medium.
- Utilization of Palmitic Acid by Mycobacterium avium
- INTRACELLULAR CONJUGATION AND DETOXIFICATION OF PALMITIC ACID BY MYCOBACTERIUM TUBERCULOSIS1
- An aqueous mounting medium.
- Effect of Palmitic Acid Utilization on Cell Division in Mycobacterium avium
