Oxygen Regulation of the ccoN Gene Encoding a Component of the cbb3 Oxidase in Rhodobacter sphaeroides 2.4.1T: Involvement of the FnrL Protein

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FONTE

American Society for Microbiology

RESUMO

The ccoNOQP gene cluster of Rhodobacter sphaeroides 2.4.1T encodes a cbb3 cytochrome oxidase which is utilized in oxygen-limited conditions for aerobic respiration. The β-galactosidase activity of a ccoN::lacZ transcriptional fusion was low under high (30%)-oxygen and anaerobic growth conditions. Maximal ccoN::lacZ expression was observed when the oxygen concentration was lowered to 2%. In an FnrL mutant, ccoN::lacZ expression was significantly lower than in the wild-type strain, suggesting that FnrL is a positive regulator of genes encoding the cbb3 oxidase.

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