P-element-induced control mutations at the r gene of Drosophila melanogaster.

AUTOR(ES)

Tsubota, S

RESUMO

The P-M hybrid dysgenesis system was used to produce five putative regulatory mutations at the rudimentary locus, r. All five mutations were the result of insertions at the 5' end of the gene, upstream of the proposed start of transcription. All of the mutants displayed a leaky wing phenotype, and four of the mutants showed an uncoupling of the wing and female-sterility phenotypes, suggesting that they altered the normal spatial and temporal expression of the r gene. Four of the insertions were P elements. The fifth insertion, which was larger than an intact P element, consisted of a small P element connected to non-P-element DNA. Two of the mutants produced very little r transcript in adult females and were clustered 80 to 150 base pairs upstream of the start of transcription. The other three mutants had higher levels of r transcript in adult females and were clustered 440 to 500 base pairs upstream of the start of transcription. All of the data suggest that the insertions are in a 5' noncoding region of the r gene involved in the control of its spatial and temporal expression.

Documentos Relacionados

• P-element-induced interallelic gene conversion of insertions and deletions in Drosophila melanogaster.
• P-Element-Induced Recombination in Drosophila Melanogaster: Hybrid Element Insertion
• Zygotic Lethal Mutations with Maternal Effect Phenotypes in Drosophila Melanogaster. II. Loci on the Second and Third Chromosomes Identified by P-Element-Induced Mutations
• The Accumulation of P-Element-Induced Recombinants in the Germline of Male Drosophila Melanogaster
• P-Element-Induced Male Recombination and Gene Conversion in Drosophila