P Element Insertions and Rearrangements at the Singed Locus of Drosophila Melanogaster

AUTOR(ES)

Roiha, H.

RESUMO

DNA from the singed gene of Drosophila melanogaster was isolated using an inversion between a previously cloned P element at cytological location 17C and the hypermutable allele singed-weak. Five out of nine singed mutants examined have alterations in their DNA maps in this region. The singed locus is a hotspot for mutation during P-M hybrid dysgenesis, and we have analyzed 22 mutations induced by P-M hybrid dysgenesis. All 22 have a P element inserted within a 700-bp region. The precise positions of 10 P element insertions were determined and they define 4 sites within a 100-bp interval. During P-M hybrid dysgenesis, the singed-weak allele is destabilized, producing two classes of phenotypically altered derivatives at high frequency. In singed-weak, two defective P elements are present in a ``head-to-head'' or inverse tandem arrangement. Excision of one element results in a more extreme singed bristle phenotype while excision of the other leads to a wild-type bristle phenotype.

Documentos Relacionados

• Restriction of P-element insertions at the Notch locus of Drosophila melanogaster.
• Molecular Analysis of an Unstable P Element Insertion at the Singed Locus of Drosophila Melanogaster: Evidence for Intracistronic Transposition of a P Element
• On the specificity and effects on transcription of P-element insertions at the yellow locus of Drosophila melanogaster.
• Structure and Transcription of the Singed Locus of Drosophila Melanogaster
• Molecular characterization of the singed wings locus of Drosophila melanogaster