Packaging cells for avian leukosis virus-based vectors with various host ranges.
AUTOR(ES)
Cosset, F L
RESUMO
Using our previously described Haydée semipackaging cell line (F. L. Cosset, C. Legras, Y. Chebloune, P. Savatier, P. Thoraval, J. L. Thomas, J. Samarut, V. M. Nigon, and G. Verdier, J. Virol. 64:1070-1078, 1990) which produces avian leukosis virus gag and pol proteins, we have constructed packaging cells with subgroups B, C, and E envelope specificities. This allows us to produce helper-free avian leukosis virus particles carrying the lacZ reporter gene and the A, B, C, or E subgroup specificities. Titers of the recombinant lacZ virus are shown to be dependent upon the type of the env subgroup and the target avian cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=289136Documentos Relacionados
- Generation of a helper cell line for packaging avian leukosis virus-based vectors.
- Development of avian sarcoma and leukosis virus-based vector-packaging cell lines.
- An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells
- Expression of transduced genes in mice generated by infecting blastocysts with avian leukosis virus-based retroviral vectors.
- A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes.