Partition functions of unit-copy plasmids can stabilize the maintenance of plasmid pBR322 at low copy number.
AUTOR(ES)
Austin, S
RESUMO
The maintenance of plasmid pBR322 is highly unstable in a polA12 strain of Escherichia coli at 29 degrees C due to severely reduced copy number. Under these conditions, introduction of the par (partition) locus of plasmid P1 or the par (sop) region of F into pBR322 stabilizes it. A region with similar activity was detected in the P7 plasmid. The activity of the P1 par locus was dependent on the P1 parA gene product and was sensitive to par-specified incompatibility.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213585Documentos Relacionados
- Maintenance of plasmids pBR322 and pUC8 in nonculturable Escherichia coli in the marine environment.
- A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect.
- Conservative integration of bacteriophage Mu DNA into pBR322 plasmid.
- Organization of transcriptional signals in plasmids pBR322 and pACYC184.
- Escherichia coli factor Y sites of plasmid pBR322 can function as origins of DNA replication.
