PCR engineering of viral quasispecies: a new method to preserve and manipulate genetic diversity of RNA virus populations.
AUTOR(ES)
Chumakov, K M
RESUMO
A PCR-based method for the controlled manipulation of individual genomic sites of poliovirus with concomitant preservation of the sequence heterogeneity of the rest of the genome is proposed. The new approach can be used for the creation of stable DNA repositories of populations of extremely heterogenous RNA viruses and may have implications for live vaccine technology.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=190799Documentos Relacionados
- Rabies virus quasispecies: Implications for pathogenesis
- Genetic Diversity in RNA Virus Quasispecies Is Controlled by Host-Virus Interactions
- A new computational method for detection of chimeric 16S rRNA artifacts generated by PCR amplification from mixed bacterial populations.
- Biochemical fingerprinting of water coliform bacteria, a new method for measuring phenotypic diversity and for comparing different bacterial populations.
- Quasispecies in wild-type tula hantavirus populations.
