Perfil de Quimiocinas em Indivíduos Portadores de Lesão Ocular Decorrente da Infecção pelo Toxoplasma gondii

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The high prevalence of toxoplasmosis at Campos dos Goytacazes city is recognized as the highest described in literature, where the prevalence of ocular disease is situated from 6 to 14% for the seropositive population. The role of chemokines and their receptors in the ocular pathology process from infectious diseases has not been well studied. In this context, we proposed to study the chemokine production profile, such as IP-10/CXCL10, MCP-1/CCL2, MIG/CXCL9, RANTES/CCL5 and IL-8/CXCL8 in patients with toxoplasmic rethinochoroiditi associating the production profile with the development of injuries in the retinal tissue. A total of 83 patients, seronegative or seropositive for toxoplasmosis, with ocular lesions or not, were involved in this study. Peripherical Blood Mononuclear Cells (PBMC) were then maintained in culture in the absence or presence of T. gondii soluble antigens (STAg) and Staphylococcal Enterotoxin B (SEB) stimulus. Cell culture supernatants collected in different days after the beginning of the culture assay were assessed regarding the chemokines production. IP-10/CXCL10, MIG/CXCL9 and MCP-1/CCL2 presented higher levels in seropositive individuals for toxoplasmosis than seronegative, when stimulated with STAg. Seropositive patients with ocular lesions presented the highest level of IP-10/CXCL10, MIG/CXCL9 production. The production of IP-10/CXCL10, MIG/CXCL9 and MCP-1/CCL2 was rather higher in patients with type A lesion (severe injury), suggesting involvement of these molecules in the ocular disease caused by infection with T. gondii. Furthermore, individuals with type A lesion presented lower production of IL-8/CXCL8 compared with patients without ocular damage and patients with type C lesion (mild injury), suggesting the possible protective role of chemokine in the toxoplasmic ocular disease. The hypothetical evolution of ocular lesions from type C to B type lesions seems to be related to reduced production of IP-10/CXCL10, MIG/CXCL9 and MCP-1/CCL2, fact that can compromise the trafficking of cells important in controlling the infection, such as Th1 cells and NK. A increased number of subjects to be studied and the following of studied patients concerning the clinical changes in association with chemokine production aspects might be crucial for the better understanding of toxoplasmosis ocular manifestations. A better knowledge of the role of chemokines in ocular diseases may signal new therapeutic prospects, which contribute to the control of inflammatory processes in the eye tissue.

ASSUNTO(S)

imunologia quimiocinas chemokines retinocoroidite rethinochoroiditi toxoplasma gondii toxoplasma gondii

ACESSO AO ARTIGO

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