Persistence of intestinal antibody response to heterologous rotavirus infection in a murine model beyond 1 year.
AUTOR(ES)
Shaw, R D
RESUMO
We used an ELISPOT (enzyme-linked immunosorbent spot) assay to quantitate the long-term rotavirus-specific intestinal antibody response in a murine model. The frequency of murine intestinal antibody-secreting cells (ASCs) was followed for a period of 1 year after a single dose of rhesus rotavirus (10(6) PFU) was administered at 10 days of age. Some animals were boosted at that time with a second dose. One year after infection, virus-specific ASCs declined from acute-phase levels, but they were still present at significant levels (1.32 x 10(4) virus-specific ASCs per 10(6) intestinal mononuclear cells; approximately 17% of the previously reported response at 1 month after infection). A booster dose 1 year after the primary infection produced a 100% increase in virus-specific ASCs but did not restore the response to that of the primary infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=262733Documentos Relacionados
- Murine intestinal antibody response to heterologous rotavirus infection.
- VP4-specific intestinal antibody response to rotavirus in a murine model of heterotypic infection.
- Complement-fixing antibody response to rotavirus infection.
- Serologic and mucosal immune response to rotavirus infection in the rabbit model.
- Antibody response to Campylobacter coli in children during intestinal infection and carriage.
