Perturbation of the T cell repertoire in rheumatoid arthritis
AUTOR(ES)
Wagner, Ulf G.
FONTE
The National Academy of Sciences
RESUMO
Aberrations in the T cell repertoire with the emergence of oligoclonal populations have been described in patients with rheumatoid arthritis (RA). However, the extent of the repertoire perturbations as well as the underlying mechanisms are not known. We now have examined the diversity of the peripheral CD4 T cell repertoire by determining the frequencies of arbitrarily selected T cell receptor (TCR) β-chain sequences. Healthy individuals displayed a highly diverse repertoire, with a median frequency of individual TCR β-chain sequences of 1 in 2.4 × 107 CD4 T cells. In RA patients, the median TCR β-chain frequency was increased 10-fold, indicating marked contraction of the repertoire (P < 0.001). The loss in TCR diversity was not limited to CD4 memory T cells but also involved the compartment of naive T cells, suggesting that it reflected an abnormality in T cell repertoire formation and not a consequence of antigen recognition in the synovium. Also, control patients with chronic inflammatory disease such as hepatitis C expressed a diverse repertoire indistinguishable from that of normals. Telomere length studies indicated an increased replicative history of peripheral CD4 T cells in RA patients, suggesting an enhanced turnover within the CD4 compartment. Compared with age-matched controls, terminal restriction fragment sizes were 1.7 kilobases shorter (P < 0.001). These data demonstrate an altered CD4 T cell homeostasis in RA that may contribute to the autoimmune response as well as to the immunodeficiency in these patients.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=24393Documentos Relacionados
- The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.
- Dominant clonotypes in the repertoire of peripheral CD4+ T cells in rheumatoid arthritis.
- Biased T-Cell Antigen Receptor Repertoire in Lyme Arthritis
- Clonal expansion is a characteristic feature of the B-cell repertoire of patients with rheumatoid arthritis
- T cell homeostasis in patients with rheumatoid arthritis