Physical analysis of phr gene transcription in Escherichia coli K-12.
AUTOR(ES)
Lorence, M C
RESUMO
The phr gene of Escherichia coli K-12 encodes the light-dependent, DNA repair enzyme photolyase, which removes UV light-induced pyrimidine dimers from cellular DNA. From Southern hybridization analysis of several strains containing successively extended phr deletions, we have determined the direction of transcription of the phr gene on the E. coli K-12 chromosome. Northern (RNA) hybridization analysis suggests that the phr gene is cotranscribed with a previously identified gene of unknown function (orf169) into two messages of different lengths. S1 nuclease mapping analysis indicates that the two transcripts share a single termination site but initiate at two different sites. Finally, we have determined that the presence of orf169 is not necessary for phr gene activity in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=526844Documentos Relacionados
- Photoreactivation in phr mutants of Escherichia coli K-12.
- Physical characterization of the cloned protease III gene from Escherichia coli K-12.
- Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12.
- Mutational analysis of nitrate regulatory gene narL in Escherichia coli K-12.
- Genetic analysis of the recJ gene of Escherichia coli K-12.