Physical and Comparative Mapping of Distal Mouse Chromosome 16
AUTOR(ES)
Cabin, Deborah E.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Distal mouse Chromosome 16 (Chr. 16) includes a region of conserved linkage with human Chromosome 21 (Chr. 21). Mouse models of Down syndrome based on trisomy of distal Chr. 16 have several phenotypes similar to those seen in human patients and have proven useful for correlating dosage imbalance of specific genes with specific developmental anomalies. The degree to which such findings can be related to Down syndrome depends on how well the conserved synteny is maintained. Twenty-four genes have been mapped in both species and there are no discordancies, but the region could carry hundreds of genes. Comparative sequence represents the ultimate comparative map and will aid in identification of genes and their regulatory sequences. A physical map of the distal 4.5 Mb of Chr. 16 has been assembled as an essential step toward a map of sequence-ready templates. The map consists of 51 YACs and 15 BACs and includes 18 transcripts, 9 of which are mapped for the first time in mouse, and 3 of which are, for the first time, described in either species. YAC fragmentation was used to precisely localize the 49 markers on the map. Comparison of this physical map with that of the corresponding region on Chr. 21 shows conservation not only of gene order but of size in the 3 Mb from Cbr1 to Ets2; distal to Ets2, the human map is expanded.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310775Documentos Relacionados
- Comparative physical mapping links conservation of microsynteny to chromosome structure and recombination in grasses
- Comparative mapping of DNA markers from the familial Alzheimer disease and Down syndrome regions of human chromosome 21 to mouse chromosomes 16 and 17.
- Identification of a cellular receptor for mouse mammary tumor virus and mapping of its gene to chromosome 16.
- Physical analysis and mapping of the Mycoplasma pneumoniae chromosome.
- Evidence for the evolutionary origin of human chromosome 21 from comparative gene mapping in the cow and mouse.