Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome.
AUTOR(ES)
Itaya, M
RESUMO
The precise physical locations of the oriC region and the region for type II DNA binding to the membrane on the Bacillus subtilis 168 chromosome were determined. The DNA regions were physically mapped by creating new restriction sites (NotI and SfiI) within these regions. The physical distance between oriC and the type II DNA-binding region was verified with the creation of a novel sequence cleaved by endonuclease I-SceI in each of the above regions. Complete removal of the defined type II membrane-binding region produced no noticeable phenotype.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206389Documentos Relacionados
- Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome.
- Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
- Characterization of the Escherichia coli membrane domain responsible for binding oriC DNA.
- Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.
- Effects of the Chromosome Partitioning Protein Spo0J (ParB) on oriC Positioning and Replication Initiation in Bacillus subtilis
