Physical map and set of overlapping cosmid clones representing the genome of the archaeon Halobacterium sp. GRB.
AUTOR(ES)
St Jean, A
RESUMO
We have constructed a complete, five-enzyme restriction map of the genome of the archaeon Halobacterium sp. GRB, based on a set of 84 overlapping cosmid clones. Fewer than 30 kbp, in three gaps, remain uncloned. The genome consists of five replicons: a chromosome (2038 kbp) and four plasmids (305, 90, 37, and 1.8 kbp). The genome of Halobacterium sp. GRB is similar in style to other halobacterial genomes by being partitioned among multiple replicons and by being mosaic in terms of nucleotide composition. It is unlike other halobacterial genomes, however, in lacking multicopy families of insertion sequences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308008Documentos Relacionados
- Completion of the detailed restriction map of the E. coli genome by the isolation of overlapping cosmid clones.
- Novobiocin induces accumulation of a single strand of plasmid pGRB-1 in the archaebacterium Halobacterium GRB.
- Towards a physical map of the Drosophila melanogaster genome: mapping of cosmid clones within defined genomic divisions.
- Nucleotide sequence of a high copy number plasmid from Halobacterium strain GRB.
- Physical genome map of the unicellular cyanobacterium Synechococcus sp. strain PCC 7002.