Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII.

AUTOR(ES)

Summers, J

RESUMO

Digestion of polyoma viral DNA with a restriction enzyme from Haemophilus aegyptius generates at least 22 unique fragments. The fragments have been characterized with respect to size and physical order on the polyoma genome, and the 5' to 3' orientation of the (+) and (-) strands has been determined. A method for specific radiolabeling of adjacent fragments was employed to establish the fragment order. This technique may be useful for ordering the fragments produced by digestion of complex DNAs.

Documentos Relacionados

• Specific Fragments of φX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius, Endonuclease Z 1
• Analysis of Simian Virus 40 DNA with the Restriction Enzyme of Haemophilus aegyptius, Endonuclease Z
• Cleavage map of BK virus DNA with restriction endonucleases MboI and HaeIII.
• Physical map and strand polarity of specific fragments of adenovirus-associated virus DNA produced by endonuclease R-EcoRI.
• Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.