Physicochemical Properties of the Vi Antigen Before and After Mild Alkaline Hydrolysis
AUTOR(ES)
Jarvis, Francis G.
RESUMO
Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 × 106. Mild alkaline hydrolysis completely removed O-acetyl, reduced the molecular weight to 1.0 × 105, and lowered the intrinsic viscosity from 8.0 deciliters/g to approximately 0.5 deciliter/g. Infrared spectra of the two antigen preparations were also compared.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=276839Documentos Relacionados
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