PLAQUE ASSAY FOR PSITTACOSIS VIRUS IN MONOLAYERS OF CHICK EMBRYO FIBROBLASTS

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Piraino, F. (City of Milwaukee Health Department, Milwaukee, Wis.), and C. Abel. Plaque assay for psittacosis virus in monolayers of chick embryo fibroblasts. J. Bacteriol. 87:1503–1511. 1964.—A tissue culture system for the assay of 6BC psittacosis virus by plaque production on chick embryo fibroblast cells is described. Experiments are given which characterize the specificity of the reaction and identify factors found to cause variations in plaque counts. The statistical data obtained indicate this assay to be a precise and highly reliable procedure for the detection of 6BC virus and antibody.

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