Plasmid maintenance functions encoded on Dictyostelium discoideum nuclear plasmid Ddp1.
AUTOR(ES)
Hughes, J E
RESUMO
All of the plasmid-carried genes expressed during vegetative growth are essential for long-term maintenance of plasmid Ddp1 in the nucleus of Dictyostelium discoideum. Deletion of Ddp1 genes expressed only during development had no detectable effect on plasmid maintenance. Deletion of vegetatively expressed genes, either singly or in pairs, resulted in (i) a rapid loss of plasmid from cells grown in the absence of selection for plasmid retention, (ii) variation in the proportion of monomer to multimer forms of the plasmid molecules, and/or (iii) abnormalities in plasmid copy number. At least two plasmid-encoded gene products influence patterns of expression of plasmid genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=359138Documentos Relacionados
- Developmentally regulated transcription of Dictyostelium discoideum plasmid Ddp1
- The extrachromosomal replication of Dictyostelium plasmid Ddp2 requires a cis-acting element and a plasmid-encoded trans-acting factor.
- The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.
- Identification of an endogenous plasmid in Dictyostelium discoideum
- A lysine-rich protein functions as an H1 histone in Dictyostelium discoideum chromatin.