Plasmid pT181 replication is regulated by two countertranscripts.
AUTOR(ES)
Kumar, C C
RESUMO
A transcription map of the replication control region of the Staphylococcus aureus plasmid pT181 has been constructed. Two major leftward transcripts, RNA III and RNA IV, start at positions 339 and 413, respectively. These two RNAs can serve as mRNAs for a plasmid-specific replication protein RepC. Two short rightward transcripts, RNA I and RNA II, approximately 85 and 150 nucleotides long, respectively, start at position 246. These rightward transcripts (referred to as countertranscripts) do not appear to be translated but act directly as negative regulators of plasmid replication, probably by interfering with translation of the RepC mRNAs. There is no significant base sequence homology among the countertranscripts of pT181, ColE1, and R1/NR1/R6-5, suggesting that the structural parallelism has risen by convergent molecular evolution.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=397100Documentos Relacionados
- Staphylococcus aureus chromosomal mutation plaC1 amplifies plasmid pT181 by depressing synthesis of its negative-effector countertranscripts.
- Intermediates in plasmid pT181 DNA replication.
- Replication of single-stranded plasmid pT181 DNA in vitro.
- Control of pT181 replication I. The pT181 copy control function acts by inhibiting the synthesis of a replication protein.
- Replication of plasmid pT181 DNA in vitro: requirement for a plasmid-encoded product.