Plasmid Replication and the Induced Synthesis of Colicins E1 and E2 in Escherichia coli

AUTOR(ES)

Durkacz, Barbara W.

RESUMO

Induction of colicins E1 and E2 in Escherichia coli occurs when plasmid synthesis has been inhibited either by nalidixic acid or by lack of deoxyribonucleic acid polymerase I. Moreover, colicin E1 and E2 synthesis induced by mitomycin C and exposure to chloramphenicol is not associated with a large increase in circular plasmid deoxyribonucleic acid. The mean plasmid content of cells in populations having a low spontaneous frequency of colicin-producing cells because of growth at low temperature or because of the presence of recA− or crp− alleles, is not significantly different to that in wild-type cells grown at 37 C.

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