Poliovirus-induced Cellular Injury
AUTOR(ES)
Blackman, Kenneth E.
RESUMO
Protein leakage was used as a quantitative measure of poliovirus-induced cellular injury under suspended cell culture conditions. The requirements for protein leakage were studied in detail and it was established that events early in the infectious cycle which depend upon viral protein synthesis were responsible for cell damage. Extralysosomal β-glucuronidase appeared in infected cells before the onset of protein leakage and release of newly synthesized virus. Hydrocortisone treatment of infected cells resulted in only a slight delay in the release of β-glucuronidase from lysosomes and protein and virus from cells. These results suggest that events associated with poliovirus synthesis trigger the release of lysosomal hydrolases which in turn injure the plasma membrane, allowing cytoplasmic proteins and virus to leak out of the cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375858Documentos Relacionados
- Poliovirus-induced alterations in HeLa cell membrane functions.
- Susceptibility and resistance to poliovirus-induced paralysis of inbred mouse strains.
- Poliovirus-induced inhibition of polypeptide initiation in vitro on native polyribosomes.
- Differential Effect of Phleomycin on the Infectivity of Poliovirus and Poliovirus-Induced Ribonucleic Acids
- Relationship of eukaryotic initiation factor 3 to poliovirus-induced p220 cleavage activity.