Polyamine Stimulation of Nucleic Acid Synthesis in an Uninfected and Phage-Infected Polyamine Auxotroph of Escherichia coli K12

AUTOR(ES)

Dion, Arnold S.

RESUMO

The addition of arginine to cultures of Escherichia coli K12 deficient in agmatine ureohydrolase (EC 3.5.3.7) results in polyamine depletion and a striking inhibition of nucleic acid accumulation and growth. The omission of lysine from these cultures leads to a further decrease in growth rate and nucleic acid synthesis. In arginine-inhibited cells the addition of putrescine or spermidine, in the presence or absence of lysine, restores the control rate of growth and nucleic acid accumulation. Under the same conditions of arginine inhibition in the absence of lysine, the addition of cadeverine alone stimulates growth rate and RNA synthesis. The addition of lysine to polyamine-depleted cultures results in cadaverine production and in the appearance of a new spermidine analogue, containing lysine carbon. The new compound has been identified as N-3-aminopropyl-1,5-diaminopentane.

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