Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.
AUTOR(ES)
Smith, C J
RESUMO
A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214267Documentos Relacionados
- Transformation of Bacillus polymyxa with plasmid DNA.
- Transformation of Azotobacter vinelandii with plasmid DNA.
- Polyethylene glycol-dependent transfection of Acholeplasma laidlawii with mycoplasma virus L2 DNA.
- Tetracycline-inducible transfer of tetracycline resistance in Bacteroides fragilis in the absence of detectable plasmid DNA.
- Transformation of Rhizobium meliloti 41 with plasmid DNA.
