Polynucleotide Ligase in Bacteriophage T4d Recombination
AUTOR(ES)
Krisch, H. M.
RESUMO
Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective) infection. In all of these cases recombination under standard cross conditions occurs early in the vegetative cycle. The increased recombination in ligase-deficient rII infection is reduced in a bacterial strain which produces greater than normal levels of host ligase. These results indicate that ligase has a crucial role not only in the replication of DNA but also in recombination. The level of ligase may determine whether DNA replication occurs with or without concomitant recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1212820Documentos Relacionados
- Replication and Recombination in Ligase-Deficient rII Bacteriophage T4D
- Dominance in Bacteriophage T4d
- Variations in Genetic Recombination Due to Amber Mutations in T4D Bacteriophage
- Replication and Recombination of Gene 59 Mutant of Bacteriophage T4D
- Region-Specific Recombination in Phage T4. III. the Effect of Host Mutations on Glucosylation-Dependent Recombination in Bacteriophage T4d