Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
AUTOR(ES)
Raptis, L
RESUMO
Polyomavirus middle tumor antigen (mT) was expressed in a line of mouse NIH 3T3 cells under control of the dexamethasone-regulatable mouse mammary tumor virus promotor. Contrary to rat F111 cells which were rendered anchorage independent by mT expression alone (L. Raptis, H. Lamfrom, and T.L. Benjamin, Mol. Cell. Biol. 5:2476-2487, 1985), mT-producing NIH 3T3 cells were unable to grow in agar even after full mT induction. The mT:pp60c-src-associated phosphatidylinositol kinase was activated in these cells to a degree similar to that in fully transformed cells expressing the small and large T antigens, in addition to mT. We therefore propose that the stimulation of this phosphatidylinositol kinase, although apparently necessary, is not sufficient for transformation of NIH 3T3 cells by polyomavirus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=247747Documentos Relacionados
- Pleiotrophin transforms NIH 3T3 cells and induces tumors in nude mice.
- Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells.
- Isolation and characterization of NIH 3T3 cells expressing polyomavirus small T antigen.
- Ligand activation of overexpressed epidermal growth factor receptors transforms NIH 3T3 mouse fibroblasts.
- Analysis of simian virus 40 small t antigen-induced progression of rat F111 cells minimally transformed by large T antigen.