Polysome Metabolism in Escherichia coli: Amicetin, an Antibiotic That Stabilizes Polysomes1
AUTOR(ES)
Ennis, Herbert L.
RESUMO
Amicetin, an aminohexosepyrimidine nucleoside antibiotic, inhibited protein synthesis in intact bacterial cells and in cell-free extracts derived from these cells. At concentrations of antibiotic which rapidly and completely inhibited protein synthesis, the polysomes in the cell were stabilized. Amicetin also inhibited the breakdown of polysomes induced by the streptogramin A antibiotics, rifampin, or puromycin, antibiotics that require peptide bond formation for expression of their action on polysomes. Thus, amicetin is another addition to the list of drugs which may be useful in studying polysome and messenger ribonucleic acid metabolism in vivo. An in vitro system is described which may be generally useful in studying polysome metabolism when antibiotic-sensitive strains are not available.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=444193Documentos Relacionados
- Polysome Metabolism in Escherichia coli: Effect of Antibiotics on Polysome Stability
- A gratuitous inducer of cat-86, amicetin, inhibits bacterial peptidyl transferase.
- Streptomycin Dependence in Escherichia coli: Effects of Antibiotic Deprivation on Ribosomes
- Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations1
- Microcin 15m from Escherichia coli: mechanism of antibiotic action.