POROSITY OF ISOLATED CELL WALLS OF SACCHAROMYCES CEREVISIAE AND BACILLUS MEGATERIUM
AUTOR(ES)
Gerhardt, Philipp
RESUMO
Gerhardt, Philipp (The University of Michigan, Ann Arbor), and Jean A. Judge. Porosity of isolated cell walls of a yeast and a bacillus. J. Bacteriol. 87:945–951. 1964.—Decagram masses of cell walls were isolated from Saccharomyces cerevisiae and Bacillus megaterium; their porosity was examined by measuring the extent of uptake with polyethylene glycols and dextrans varying in molecular weight from 62 to 2,000,000. The results indicated that both walls are heteroporous. The near equality of extrapolated water-uptake values and determined moisture contents suggested that water in the cell walls is mainly free for distribution of solutes. Polymers with molecular weights of 4,500 and above were excluded by the yeast walls, and those with molecular weights of 57,000 were excluded by the bacillus walls; from these results, maximal openings of 36 and 107 A, respectively, were calculated. Electron micrographs of shadowed, stained, and sectioned walls revealed fine structure not inconsistent with heteroporosity, but the predicted openings were not seen. Altogether, in structure and permeability behavior, the cell walls were like a random meshwork of cross-linked macromolecular strands.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=277115Documentos Relacionados
- Density, porosity, and structure of dried cell walls isolated from Bacillus megaterium and Saccharomyces cerevisiae.
- Influence of Magnesium Ions on Porosity of the Bacillus megaterium Cell Wall and Membrane
- ANTIGENIC ANALYSIS OF CELL STRUCTURES ISOLATED FROM BACILLUS MEGATERIUM1
- Effect of β-Lysin on Isolated Cell Walls and Protoplasts of Bacillus subtilis
- Saccharomyces cerevisiae mannoproteins form an external cell wall layer that determines wall porosity.
