Post-transcriptional suppression of globin gene expression in cells transformed by avian erythroblastosis virus.
AUTOR(ES)
Therwath, A
RESUMO
Cells transformed by avian erythroblastosis virus were grown in vitro for up to 5 months. After a few days in culture, synthesis of hemoglobin was undetectable and could not be induced by dimethyl sulfoxide. As shown by globin cDNA hybridization to nuclear and cytoplasmic RNA carried to Crot values of 10(5) moles of nucleotide per liter X sec, globin genes in these cells are transcribed into pre-mRNA, but no trace of globin mRNA appears in the cytoplasm. The implications of this observation for schemes of post-transcriptional regulations and viral transformation are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392869Documentos Relacionados
- Alteration of cellular gene expression in adenovirus transformed cells by post-transcriptional mechanisms.
- Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA
- Transcriptional and post-transcriptional regulation of globin gene accumulation in murine erythroleukemia cells.
- Macromolecular Synthesis in Cells Infected by Frog Virus 3. VII. Transcriptional and Post-Transcriptional Regulation of Virus Gene Expression
- Heterologous expression of plant virus genes that suppress post-transcriptional gene silencing results in suppression of RNA interference in Drosophila cells
