Posttranscriptional autoregulation of Escherichia coli threonyl tRNA synthetase expression in vivo.
AUTOR(ES)
Butler, J S
RESUMO
Five mutations in thrS, the gene for threonyl-tRNA synthetase, have been characterized, and the sites of the mutations have been localized to different regions of the thrS gene by recombination with M13 phage carrying portions of the thrS gene. Quantitative immunoblotting shows that some of these mutations cause the overproduction of structurally altered threonyl-tRNA synthetase in vivo. The amounts of in vivo thrS mRNA as measured by quantitative hybridization are, however, the same as wild-type levels for each mutant. These results demonstrate that the expression of threonyl-tRNA synthetase is autoregulated at the posttranscriptional level in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214389Documentos Relacionados
- Escherichia coli Mutants Overproducing Phenylalanyl- and Threonyl-tRNA Synthetase
- Molecular mimicry in translational control of E. coli threonyl-tRNA synthetase gene. Competitive inhibition in tRNA aminoacylation and operator-repressor recognition switch using tRNA identity rules.
- Molecular recognition of tRNA(Pro) by Escherichia coli proline tRNA synthetase in vitro.
- Functional communication in the recognition of tRNA by Escherichia coli glutaminyl-tRNA synthetase.
- Temporal appearance of bacteriophage T4-modified valyl tRNA synthetase in Escherichia coli.
