Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation.
AUTOR(ES)
Ingelbrecht, I
RESUMO
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=45049Documentos Relacionados
- Position-Dependent Methylation and Transcriptional Silencing of Transgenes in Inverted T-DNA Repeats: Implications for Posttranscriptional Silencing of Homologous Host Genes in Plants
- RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing.
- Activity of the transposon Tam3 in Antirrhinum and tobacco: possible role of DNA methylation.
- The sequence specificity of vertebrate DNA methylation.
- Free radical adducts induce alterations in DNA cytosine methylation.