Potassium Uptake During Microcyst Formation in Myxococcus xanthus

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RESUMO

The kinetics of 42K uptake by Myxococcus xanthus during vegetative growth and microcyst formation were determined. In the medium studied, growing cells concentrated potassium about 100-fold, yielding an intracellular concentration of 147 mm. The influx of K+ in growing cells was 17 ± 3 pmoles of K+/cm2 min. About 5 hr after induction of vegetative cells to microcysts, the K+ influx decreased and the intracellular concentration fell. By 18 hr after induction, there was no measurable influx of K+, and the intracellular concentration of potassium was less than 29 mm. There was, however, considerable binding of K+ to the “surface” of microcysts. It is postulated that the greatly reduced intracellular concentration of potassium helps to maintain the microcyst in its dormant state and protects it against enzymatic break-down.

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