Precise nucleosome positioning in the promoter of the chicken beta A globin gene.
AUTOR(ES)
Kefalas, P
RESUMO
Histone octamers were reconstituted onto 5' end-labelled DNA fragments derived from the promoter region of the chicken beta A globin gene. The location of the reconstituted histone octamer with respect to the DNA sequence of each fragment was assessed by Exonuclease III digestion of purified nucleosome monomers. By this approach we have found a strong preference for histone octamers to be positioned over nucleotides -206 to -62 relative to the gene cap site. This stretch of DNA contains all those 5' beta globin sequences which, by DNase footprinting, bind specific protein factors and incorporates three promoter consensus sequence motifs. The upstream terminal 32 base pairs of this DNA segment contains the binding sites for the erythrocyte specific G-string binding protein and transcription factor Spl and appears to be relatively weakly bound to the histone octamer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=334675Documentos Relacionados
- Periodicity of strong nucleosome positioning sites around the chicken adult beta-globin gene may encode regularly spaced chromatin.
- Transcription termination at the chicken beta H-globin gene.
- The promoter and enhancer of the inactive chicken beta-globin gene contains precisely positioned nucleosomes.
- Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
- Analysis of the tissue-specific enhancer at the 3' end of the chicken adult beta-globin gene.
