Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.
AUTOR(ES)
Lomedico, P T
RESUMO
Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation system. On sodium dodecyl sulfate polyacrylamide gels, this polypeptide (12,000-13,000 daltons) is larger than proinsulin (9,000 daltons).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342908Documentos Relacionados
- Cell-free translation of influenza virus mRNA.
- Translation of Virus mRNA: Synthesis of Bacteriophage Qβ Proteins in a Cell-Free Extract from Wheat Embryo
- Regulation of protein synthesis in rat ventral prostate: Cell-free translation of mRNA
- Cell-free translation and partial characterization of mRNA coding for enkephalin-precursor protein.
- Selective inhibition of cell-free translation by oligonucleotides targeted to a mRNA hairpin structure.