Preservation of nasopharyngeal smears for fluorescent antibody detection of Bordetella pertussis.
AUTOR(ES)
Harris, P P
RESUMO
Smears from throat and nasal washings seeded with Bordetella pertussis were either treated with aprotinin (a protease inhibitor) or fixed with 1 or 10% Formalin. These smears were stored at 23, 4, and -70 degrees C. Smears were removed and stained with a fluorescent antibody conjugate for B. pertussis at intervals during 22 days of storage. Results indicate that treatment of smears with 4 or 8 U of aprotinin per ml preserved fluorescent antibody staining qualities of B. pertussis for 22 days; fixation with either concentration of Formalin was unsatisfactory.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273702Documentos Relacionados
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