Pretreatment with Urea-Hydrochloric Acid Enhances the Isolation of Helicobacter pylori from Contaminated Specimens

AUTOR(ES)

Song, Qunsheng

FONTE

American Society for Microbiology

RESUMO

Human saliva seeded with H. pylori was incubated in urea-HCl and then cultured on nonselective media. Pretreatment with 0.06 N HCl–0.08 M urea for 5 min at 37°C resulted in reproducible isolation of H. pylori, even at low inocula (≤102 CFU/ml of saliva), despite the presence of large numbers of contaminating organisms.

Documentos Relacionados

• Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens.
• Ribotyping of Helicobacter pylori from clinical specimens.
• Isolation of Helicobacter pylori from saliva.
• Intragastric urea hydrolysis in children infected with Helicobacter pylori.
• Autodisplay: Efficacious Surface Exposure of Antigenic UreA Fragments from Helicobacter pylori in Salmonella Vaccine Strains