Prevention of human immunodeficiency virus type 1 integrase expression in Escherichia coli by a ribozyme.
AUTOR(ES)
Sioud, M
RESUMO
Ribozymes are potentially very powerful agents for perturbing intracellular gene expression. However, pilot experiments in eukaryotes have met with mixed success. We now report that a ribozyme designed to cleave the integrase gene of the human immunodeficiency virus (HIV), when transcribed from a plasmid in Escherichia coli, led to destruction of integrase RNA and complete blockage of integrase protein synthesis. These results indicate that ribozymes can be used to study intracellular gene expression in bacteria and that the HIV-1 integrase gene may be a useful target for therapeutic ribozymes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=52283Documentos Relacionados
- Inhibition of human immunodeficiency virus type 1 expression by a hairpin ribozyme.
- A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1.
- Efficient expression by an alphavirus replicon of a functional ribozyme targeted to human immunodeficiency virus type 1.
- Directed integration of viral DNA mediated by fusion proteins consisting of human immunodeficiency virus type 1 integrase and Escherichia coli LexA protein.
- Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations.
