Primary isolation of Mycobacterium avium complex-serotype 6 on blood agar.

AUTOR(ES)

Thigpen, J E

RESUMO

Mycobacterium avium complex-serotype 6 was isolated in pure culture on blood agar plates from inocula taken from the heart blood, lungs, liver, kidneys, and spleen of a naturally infected captive female opossum (Didelphis marsupialis virginiana). Repeat cultures from stored tissues and transfer of colonies from original blood agar plates revealed that the mycobacterium grew on tryptose, brilliant green, eosin-methylene blue. Sabouraud glucose, and mycobiotic agar plates and in Fletcher leptospira medium. The cultural, biochemical, and serological characteristics of the test isolate were compared with other mycobacteria. This is the first report to describe the primary isolation of a serotype from the M. avium complex from an animal species on blood agar or in Fletcher broth. In addition, this is the second documented report describing the isolation and identification of a mycobacterial species from the American opossum.

Documentos Relacionados

• Isolation of Nocardia asteroides on buffered charcoal-yeast extract agar.
• Isolation of Vibrio parahaemolyticus from fecal specimens on mannitol salt agar.
• Effect of gaseous conditions on isolation and growth of Ureaplasma urealyticum on agar.
• Mycobacterium avium complex: significance of isolation from bone marrow culture.
• Effect of agitation of BACTEC 13A blood cultures on recovery of Mycobacterium avium complex.