Primary sequence and developmental expression of a novel Drosophila melanogaster src gene.
AUTOR(ES)
Gregory, R J
RESUMO
We have sequenced a cDNA clone for the Drosophila melanogaster gene Dsrc28C, a homolog of the vertebrate gene c-src. The cDNA contains a single open reading frame encoding a protein of 66 kilodaltons which contains features highly conserved within the src family of tyrosine protein kinases. Novel structural features of the Dsrc28C protein include a basic pI and a polyglycine domain near the amino terminus. Cell-free translation of in vitro-transcribed RNA yielded a protein of the predicted size which could be immunoprecipitated by anti-v-src antisera. RNA blot hybridization revealed that the gene is expressed predominantly during embryogenesis, in imaginal disks of third-instar larvae, and in adult females. In situ hybridization showed that expression in adult females is largely confined to nurse cells and developing oocytes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=365333Documentos Relacionados
- The nucleotide sequence of a Drosophila melanogaster enolase gene.
- Heat shock and developmental regulation of the Drosophila melanogaster hsp83 gene.
- Nucleotide sequence of a Drosophila melanogaster H1 histone gene.
- 5'-flanking sequence required for regulated expression of a muscle-specific Drosophila melanogaster actin gene.
- Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.