Primer binding sites corresponding to several tRNA species are present in DNAs of different members of the same retrovirus-like gene family (VL30).
AUTOR(ES)
Itin, A
RESUMO
We analyzed the putative tRNA primer binding site (PBS) present in several cloned copies of the murine retrovirus-like VL30 family. In the five VL30 DNA clones analyzed, we identified PBS sequences corresponding to three different tRNA species: tRNAPro, tRNAGly, and tRNAGln. The latter two PBS sequences have not been previously encountered in other retroviral or retrovirus-like systems. A unique situation was observed in which PBS sequences complementary to two different tRNA species were flanked by otherwise identical VL30 sequences. In addition, we demonstrated the use of PBS-specific synthetic oligonucleotides for the identification of the tRNA primer and their potential utility in the direct cloning of PBS-containing DNA elements.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254784Documentos Relacionados
- 'Solo' large terminal repeats (LTR) of an endogenous retrovirus-like gene family (VL30) in the mouse genome.
- Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements.
- Cleavage of tRNA within the mature tRNA sequence by the catalytic RNA of RNase P: implication for the formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles.
- Defective retrovirus-like 30S RNA species of rat and mouse cells are infectious if packaged by type C helper virus.
- Sequences homologous to retrovirus-like genes of the mouse are present in multiple copies in the Syrian hamster genome.
