Process formation in Sf9 cells induced by the expression of a microtubule-associated protein 2C-like construct.

AUTOR(ES)
RESUMO

To understand the roles of various microtubule-associated proteins (MAPs) in the development of axons and dendrites, we have expressed individual neuronal MAPs in normally rounded Sf9 host cells. We previously reported that expression of tau protein in these cells results in the elaboration of long processes containing dense bundles of microtubules (MTs). These bundles generally terminate in the hillock region of the cell body, and almost all of the MTs within the bundles are oriented with their plus ends distal to the cell body. Here we report the expression of a construct that approximates the MAP2C sequence and also induces the elaboration of processes with dense bundles of predominantly plus-end-distal MTs. Whereas tau generally results in a single process, there is a significantly greater tendency for the MAP2C-like construct to induce multiple processes. In contrast to the tau processes, the MT bundle in these processes extends far into the cell body. This latter observation suggests that MAP2C and tau have different effects on MT assembly and/or transport events in the cell. Although both of these MAPs can organize MTs that are competent to participate in process formation, the detailed organization of MTs induced by each of the two constructs is distinctive, and these differences may be relevant to axonal and dendritic differentiation.

ACESSO AO ARTIGO

Documentos Relacionados