Procollagen mRNA metabolism during the fibroblast cell cycle and its synthesis in transformed cells.
AUTOR(ES)
Parker, I
RESUMO
Procollagen mRNA was isolated from mouse embryos and used for the synthesis of a highly labelled cDNA probe complementary to collagen mRNA. This probe was used for the investigation of procollagen mRNA metabolism during the cell cycle of 3T6 mouse embryo fibroblasts in culture. Titration hybridization experiments revealed that procollagen mRNA was present throughout the cell cycle following stumulation of confluent monolayers. Procollagen mRNA levels of sparse cultures appeared similar to those of unstimulated monolayers. The fluctuating levels of collagen synthesis during the cell cycle can be ascribed to changes in the amount of collagen mRNA present. In mouse sarcoma virus transformed 3T3 cells only 20--30% of the amount of procollagen mRNA in 3T3 cells is present indicating that the decline in collagen synthesis is due to mRNA availability.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324123Documentos Relacionados
- Osteonectin mRNA: distribution in normal and transformed cells.
- Role of procollagen mRNA levels in controlling the rate of procollagen synthesis.
- Extreme instability of myc mRNA in normal and transformed human cells.
- Synthesis of globin mRNA in relation to the cell cycle during induced murine erythroleukemia differentiation.
- Control of thymidine kinase mRNA during the cell cycle.
