Production of agglutinating monoclonal antibody against antigen 8 specific for Cryptococcus neoformans serotype D.
AUTOR(ES)
Ikeda, R
RESUMO
A hybridoma (clone CRND-8) that produced agglutinating monoclonal antibody (MAb) against Cryptococcus neoformans serotype D was established by using a soluble capsular polysaccharide-keyhole limpet hemocyanin conjugate for immunization. The isotype was immunoglobulin M(kappa). Specificity was determined by cell slide agglutination and enzyme-linked immunosorbent assay (ELISA). In both tests, the MAb reacted to serotypes D and A-D but not to serotypes A, B, and C. Furthermore, the specificity of the MAb determined by ELISA was the same as that of polyclonal antibody factor serum (PAb factor) 8, which showed high-level reactivity with serotypes D and A-D. These results supported the deduced specificity of the PAb-based antigenic factor 8. A total of 15 isolates of serotypes D and A-D but no serotype A isolates reacted with the MAb in cell slide agglutination tests. CRND-8 MAb can be used in place of PAb factor 8 for serotyping C. neoformans isolates and for the analysis of the antigen 8 epitope.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170253Documentos Relacionados
- DNA typing suggests pigeon droppings as a source of pathogenic Cryptococcus neoformans serotype D.
- Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide.
- Serotyping of Cryptococcus neoformans by using a monoclonal antibody specific for capsular polysaccharide.
- Production of species-specific murine monoclonal antibodies against Cryptococcus neoformans which recognize a noncapsular exoantigen.
- Protection of mice against experimental cryptococcosis by anti-Cryptococcus neoformans monoclonal antibody.
