Production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence.
AUTOR(ES)
Waner, J L
RESUMO
Monoclonal antibodies were produced against parainfluenza virus type 3 (PI-3) and used to identify PI-3 clinical isolates in cell culture and PI-3 antigen in cells obtained from nasopharyngeal (NP) washes of patients. Two (2E9 and 4G5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton PI-3 protein, and one antibody (4E5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. The three monoclonal antibodies did not cross-react by indirect immunofluorescence (IFA) with PI-1 or PI-2 and identified by IFA 18 isolates of PI-3 in cell culture. The 2E9 antibody reacted with PI-3 antigen in cells of 8 NP wash specimens that also yielded PI-3 in cell culture. Cells from 12 specimens reactive by IFA for respiratory syncytial virus, 1 specimen yielding adenovirus in cell culture, and 5 specimens yielding influenza virus were not reactive.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=268462Documentos Relacionados
- Monoclonal antibodies against Candida tropicalis mannan: antigen detection by enzyme immunoassay and immunofluorescence.
- Diagnosis of herpes simplex virus infection by immunofluorescence.
- Diagnosis of herpes virus hominis keratitis by immunofluorescence.
- Production of monoclonal antibodies against Rickettsia massiliae and their use in antigenic and epidemiological studies.
- Use of FITC-protein A in place of fluorescein-conjugated anti-gammaglobulins for rapid virus diagnosis by immunofluorescence.
