Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M.
AUTOR(ES)
de Graaf, F K
RESUMO
Enterotoxigenic Escherichia coli strains of serogroups O9 and O101 produce an adhesive antigen, provisionally designated as F41. Production of the F41 antigen was shown to be dependent on the composition of the growth medium. A minimal salts medium or Minca medium was the most suitable medium to obtain a high production. The biosynthesis of the F41 antigen was repressed at 18 degrees C or in the presence of L-alanine. The F41 antigen was isolated from the bacteria by mechanical detachment, concentrated by precipitation with ammonium sulfate, and purified gel filtration on Sepharose CL-4B and treatment with deoxycholate. The purified F41 was composed of protein subunits with an apparent molecular weight of 29,500 on sodium dodecyl sulfate-polyacrylamide gels. The isoelectric point of the antigen was 4.6. The N-terminal amino acid sequence was determined. The F41 antigen had strong hemagglutinating activity with guinea pig and human group A erythrocytes and weaker hemagglutinating activity with horse and sheep erythrocytes. In immunoelectrophoresis at pH 8.4 the purified antigen migrated to the anode. Purified F41 antigen has a filamentous structure with an average diameter of 3.2 nm.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=351294Documentos Relacionados
- Characterization of the F41 fimbrial antigen of enterotoxigenic Escherichia coli by using monoclonal antibodies.
- Isolation and characterization of F12 adhesive fimbrial antigen from uropathogenic Escherichia coli strains.
- Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99.
- Production of fimbrial adhesins K99 and F41 by enterotoxigenic Escherichia coli as a function of growth-rate domain.
- Nucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41.